TABLE 1.
TaqMan real-time RT-PCR primers, probes, and their sequences
| Gene | Spy no. | Sequence (5′-3′)
|
||
|---|---|---|---|---|
| Forward primer | Reverse primer | TaqMan probe | ||
| fasC | 244 | CACAAAAACCAATCGTGTGGATA | CAGTCAAAAAGTGGGCTGAGTTC | 6FAM-AGCCAAACGCTTTGATGCTCAGCAG-TAMRA |
| covS | 337 | CCTGGCTTGCATGGTCCT | TGGAAAACCCACGATACTGATCT | 6FAM-TCGGTCGTGTGTATCACGACCATATCG-TAMRA |
| sycF | 529 | GCGCTTTGCGTTGAATAGG | TCCTGTTCTGTGGTGTCATGGA | 6FAM-CCACAACCAAGCCCGAAATAAAACCACT-TAMRA |
| srtK | 1082 | AATCGGTTTGGCATTTGTTAAAG | GCACCTCCTCTAGCTGGATTATTC | 6FAM-TTGCTATCAAACACGGTGGGAATCTTCAAT-TAMRA |
| ciaH | 1236 | GGTCCAGGGATAACAGATGAAGAA | CCACCTGTTTGCCGTGTTC | 6FAM-AGCTTGTCAACTCGATAAAAACGATCAAAAATCTTT-TAMRA |
| zmpR | 1556 | AAAAGAAGTATCAGCAATAGCAATGG | TGCTGAGCCAAGCTTTTTAGG | 6FAM-TCAAATCAGAATCTGCCAACCGCTCA-TAMRA |
| irr | 2027 | TCGAAAATGTATTACGCGTCTCTACT | CGCTATGTTCGGTCTCATCAAC | 6FAM-CAGATGAAAAGCGCCAAATCGGAGACTTAC-TAMRA |
| perR | 187 | GCAATGATTTAACCACCTACTATGACTT | GCAATCTTCCCACATATTTCACAA | 6FAM-CCACATTGACGTGTTGATGGCCCT-TAMRA |
| crgR | 1870 | ATATGCAAGCCTATCATCTGACATC | GGATGCTGTAATGGCAACAGT | 6FAM-AGGTTGAGTTGGAATATCGGATTGAAGTGCC-TAMRA |
| mgaa | 2019 | GCGTTTGATAGCATCAAACAAGA | CATCAAGGAGATGAACCCAGTTG | 6FAM-TCACTTTTCGACAGCCCGTTGGTGA-TAMRA |
| mgab | 2019 | TGGTGATGCTGAGATTGAGCTT | GTGCTTTTGAGATTATCRATCAAGACC | 6FAM-CCRAYATCAGGAGGCAGACAAGTAACCA-TAMRA |
| mgac | 2019 | ATGAGCTGAGAAAAACGGTGACT | AGCACCTAAAAATACTAAGCTCAGTTAACT | 6FAM-CTATTACAAGGGATACTCTGCCGTCTACGACAACA-TAMRA |
| rgg | 2042 | GATAGTAAGTCAACAAAGGAAAAGAACCT | AAATAAGTCCGCTCTGTCAGACAGT | 6FAM-TTCCTCAGTAAGAGTTGCTAATAACACCTTGACCAA-TAMRA |
| mac | 861 | AGGCCTATCACACACCTACGCT | CCCGTTAGAATCAAAGTCAGCTC | 6FAM-CGTACGCATCAACCATGTTATAAACCTGTGG-TAMRA |
| grab | 1357 | TGTTGACTCACCTATCGAACAGC | TGGAGCATTGCCAAGAAGATT | 6FAM-TCGAATTATTCCAAATGGCGGAACCTTAAC-TAMRA |
| scpA | 2010 | CGAAAGAACCTTACCGCCTAGA | TTTACAATTTCGACACGCATCAA | 6FAM-GCAATTGAGCCTCAGGCATCGCA-TAMRA |
| emm | 2018 | GCTCTTGAAAAACTTAACAAAGAGCTT | TCGCTAATTGTTCTTTGAGTGCTT | 6FAM-AAAGCTGAGCTACAAGCAAAACTTGAAGCAGA-TAMRA |
| speB | 2039 | CGCACTAAACCCTTCAGCTCTT | ACAGCACTTTGGTAACCGTTGA | 6FAM-GCCTGCGCCGCCACCAGTA-TAMRA |
| sda | 2043 | CCCAAAATGTAGGAGGTCGTG | CATTCTTGAGCTCTTTGTTCGGT | 6FAM-CCAAAAAGGCGGCATGCGCT-TAMRA |
| edin | 428 | AAAACGGCGCCATGACC | GCTGCCTTGGCCCCTTT | 6FAM-ACAGATGCGCACCTCCACCGG-TAMRA |
| bspA | 843 | AGAGCCGGTATTTTCCAAGCT | TCTCAGAGTGCGTACCTGGTTTAG | 6FAM-ATGCAGCGGCAGAAGCAGAGCAGTTA-TAMRA |
| sic | 2016 | GAGGACACCCCTCCAAGTGA | TCTTGTGGATTTTTTTGAGGAGTATG | 6FAM-CCTCGTGTGCCAGAAAAACCGCA-TAMRA |
| proS | 1962 | TGAGTTTATTATGAAAGACGGCTATAGTTTC | AATAGCTTCGTAAGCTTGACGATAATC | JOE-TCGTAGGTCACATCTAAATCTTCATAGTTG-QSY-7 |
a
Primers and probe were designed for the mga alleles in type M1 and M12 GAS strains.
b
Primers and probe were designed for the mga alleles in type M2, M4, M28, M75, M77, and M89 GAS strains.
c
Primers and probe were designed for the mga allele in type M6 GAS strains.