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Purification of recombinant Apa protein. The insert from ARRMTB65 was cloned into pRSETC as described in Materials and Methods. Lanes: 1, IPTG-induced lysate from E. coli BL21(DE3) carrying the pRSETC-65 recombinant after induction with IPTG; 2, Apa protein from lane 1 FPLC-purified by using Ni2+-NTA agarose.
