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. 2003 Apr;71(4):1929–1937. doi: 10.1128/IAI.71.4.1929-1937.2003

FIG. 4.

FIG. 4.

Animals immunized with Apa-expressing DNA, followed by a poxvirus boost, are protected against challenge with M. tuberculosis. (A) Animals immunized with APADNA/APAMVA have significantly fewer M. tuberculosis bacilli in the lungs than control animals. Challenged animals were sacrificed at the end of the 6-week observation period, and the CFU of M. tuberculosis in the spleen were enumerated. The data are given as the CFU per spleen for each animal in the groups. P values for significance between the mean CFU values for each group are shown. P values of <0.05 are significant. The lower limit of detection was 50 bacilli per organ (one CFU on a plate seeded with 100 μl of an undiluted 1% sample [5 ml] of a spleen). (B) Weekly weights of animals for 6 weeks after challenge with M. tuberculosis by the intramuscular route in the immunization groups described. Weights are plotted against time in weeks for each animal. Symbols: ▪, DNA/MVA; •, DNAAPA/MVA; ▴, DNAAPA/MVAAPA; ⧫, BCG.