. 2003 Apr;71(4):1929–1937. doi: 10.1128/IAI.71.4.1929-1937.2003

TABLE 1.

T-cell responses to total mycobacterial sonicate and the Apa protein of M. tuberculosis among PPD-positive human volunteers^a

PPD-positive healthy controls								TB patients
Serial no.	Apa protein			MTB sonicate				Serial no.	APA protein			MTB sonicate
	SI (IFN-γ)	% T-cell subset		SI (IFN-γ)	% T-cell subset				SI (IFN-γ)	% T-cell subset		SI (IFN-γ)	% T-cell subset
	SI (IFN-γ)	CD4⁺	CD8⁺	SI (IFN-γ)	CD4⁺	CD8⁺	CD4⁺		SI (IFN-γ)	CD8⁺	CD4⁺	SI (IFN-γ)	CD8⁺
HC1	7.1 (1,825)	0.40	0.30	11.3 (5,309)	1.34	0.14		P2	7.0 (947)	0.07	0.08	35.0 (4,889)	0.86	0.09
HC2	3.6 (162)	0.02	0.00	15.6 (1,127)	0.08	0.01		P7	5.5 (717)	0.06	0.12	27.0 (3,995)	0.89	0.06
HC10	6.7 (746)	0.08	0.15	30.7 (6,267)	1.31	0.14		P8	1.0 (6)	0.0	0.0	37.7 (2,374)	0.38	0.02
HC11	8.8 (4,472)	0.72	1.75	10.6 (4,374)	1.12	0.08		P9	5.5 (1,250)	0.17	0.08	19.2 (6,700)	1.35	0.09
HC12	15.2 (1,708)	0.79	1.50	10.7 (1,541)	0.20	0.18		P17	4.8 (192)	0.01	0.03	17.2 (3,724)	0.62	0.06
HC13	26.9 (7,454)	2.33	4.13	17.7 (2,510)	0.24	0.13		P18	3.6 (350)	0.08	0.04	19.0 (2,606)	0.70	0.22
HC21	10.9 (1,802)	0.32	0.28	20.8 (3,028)	0.62	0.06		P19	5.0 (287)	0.03	0.10	12.6 (1,920)	0.22	0.04
HC22	5.3 (1,448)	0.16	0.33	17.4 (3,650)	0.37	0.02		P21	1.0 (127)	0.03	0.01	9.4 (1,002)	0.16	0.02
HC31	7.0 (1,237)	0.08	0.00	36.7 (4,074)	0.36	0.02

The SI values shown were obtained by dividing the mean cpm of triplicate cultures stimulated with antigen by that of unstimulated control wells. The IFN-γ levels shown in parentheses are in picograms/milliliter and were obtained by subtracting IFN-γ levels in culture supernatants of unstimulated cells from those of cells stimulated with antigen. % T-cell subset values are shown as percentages of each T-cell subset (either CD4⁺ or CD8⁺) after deducting the background frequencies for each subset obtained in the absence of antigen. The percentages of the CD4⁺ cells shown were obtained by independent staining with antibodies to human CD4.