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. 2003 Apr;71(4):1828–1832. doi: 10.1128/IAI.71.4.1828-1832.2003

FIG. 2.

FIG. 2.

COWP (A) and SSU rRNA (B) PCR-RFLP analyses of oocysts from C. meleagridis TU1867 passaged through different host species. For COWP and SSU rRNA RFLP analysis, PCR products were digested with RsaI and AseI, respectively. Samples are from the following animal passages: human (lane 2), first mouse (lane 3), last mouse (lane 4), first pig (lane 5), last pig (lane 6), first chicken (lane 7), last chicken (lane 8), first turkey (lane 9), last turkey (lane 10), and calf (lane 11). C. parvum genotype 1 (TU502) and genotype 2 (GCH1) digested PCR products are shown in lanes 12 and 13, respectively. A negative PCR control (lane 14), uncut PCR product (lane 15), and 100-bp DNA ladder (lanes 1 and 16; Promega Corp., Madison, Wis.) are also included. The mock DNA extraction sample was negative for both PCRs (data not shown). The expected sizes of the 553-bp RsaI-digested C. meleagridis COWP fragment are 372, 147, and 34 bp, and the sizes of the 833-bp AseI-digested SSU rRNA fragment are 456, 171, 104, and 102 bp. The expected sizes of this COWP fragment are 284, 129, 106, and 34 bp for C. parvum genotype 1 and 413, 106, and 34 bp for C. parvum genotype 2. The expected sizes of the SSU rRNA fragment are 561, 104, 102, and 70 bp for C. parvum genotype 1 (837 bp, AF481962) and 628, 104, and 102 bp for genotype 2 (834 bp, AF164102).