FIG. 3.

Verification of expression site structure by Southern blotting of genomic DNA. A. phagocytophilum DNA from either strain NY18 (NY) or strain Webster (WB) was digested with the enzyme XbaI to release the 1.9-kb fragment containing the expressed msp2(p44) gene (ES) and the 1.3-kb fragment containing p44ESup1. Digested and separated DNA was hybridized with probes 1 to 4 against different regions of the expression site (see Fig. 1 for locations of probes). Molecular weight markers are in the far right lane of each blot.