FIG. 7.

The predominant msp2(p44) sequence variants in the expression site are different during in vitro growth in HL-60 and ISE6 cells. A. phagocytophilum was grown continuously in HL-60 (HH) or ISE6 (II) cells or transferred between them (HI, HIH). A total of 70 to 92 independent clones of the genomic expression site were prepared and analyzed from each of the four populations of A. phagocytophilum, as in Fig. 5. (A) RFLP analysis of expression site clones from each population. The percentage of clones with each digestion pattern is given below each gel. (B) Alignment of the CVRs of the msp2(p44) expression sites from the predominant sequence variants determined in panel A.