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. 2003 Apr;71(4):2208–2217. doi: 10.1128/IAI.71.4.2208-2217.2003

TABLE 1.

Strains and plasmids used

Strain or plasmid Relevant genotype and/or phenotypea Reference and/or source
S. aureus
    A002 Clinical S. aureus isolate MGHb; this study
    A003 Clinical S. aureus isolate 36
    A091 Clinical S. aureus isolate MGH; this study
    NCTC 8325 Wild-type strain; rsbU mutant 41; The Staphylococcal Genetic Stock Center; J. J. Iandolo
    NCTC 8325-4 Prophage-cured derivative of NCTC 8325; rsbU mutant 63 S. J. Foster
    RN6390 Prophage-cured derivative of NCTC 8325 68; A. L. Cheung
    RN6390B RN6390 reisolated in the Novick laboratory with stable alpha-hemolysin production 64; M. J. McGavin
    COL Wild-type Mcr strain; mec 75; G. B. Pier
    Newman ATCC 25904; high level of clumping factor; σB+ 32; M. Bischoff
    Reynolds Wild-type strain; type 5 capsular polysaccharide 49; J. C. Lee
    RN6911 RN6390 Δagr::tetM agr sarA+ Tcr 47; A. L. Cheung
    ALC488 RN6390 sarA::ermC agr+sarA Emr 19; A. L. Cheung
    ALC837 RN6390 hla::ermC Hla Emr 45; A. L. Cheung
    ALC842 RN6390 Δagr::tetM sarA::ermC agr sarA Tcr Emr 19; A. L. Cheung
    SP6391 RN6390B sspA::ermAB SspA Emr 72; M. J. McGavin
    IK194 Newman ΔrsbUVW sigB::ermB σB− Emr 8; M. Bischoff
    ALC1435 RN6390(pALC1420) sarA P1 promoter::gfpuv 23; A. L. Cheung
    SH1000 NCTC 8325-4 rsbU+ 40; S. J. Foster
E. coli
    OP50 Uracil auxotrophy 15; laboratory collection
    DH5α(pSMC2) DH5α containing a stable plasmid which constitutively expresses a bright mutant of A. victoria GFP; Apr 11
E. faecium
    E007 Clinical E. faecium isolate 36
B. subtilis
    PY79 Laboratory strain 85
C. elegans
    Bristol N2 Wild-type strain Laboratory strain
    AU1 esp-2(ag1) 46; D. H. Kim
    AU3 esp-8(ag3) 46; D. H. Kim
    BA1 fer-1(hc1) Laboratory strain
a

Abbreviations: Mc, methicillin; Tc, tetracycline; Em, erythromycin; Ap, ampicillin; Hla, alpha-hemolysin; SspA, V8 protease.

b

MGH, Massachusetts General Hospital.