TABLE 1.
Strains and plasmids used
| Strain or plasmid | Relevant genotype and/or phenotypea | Reference and/or source |
|---|---|---|
| S. aureus | ||
| A002 | Clinical S. aureus isolate | MGHb; this study |
| A003 | Clinical S. aureus isolate | 36 |
| A091 | Clinical S. aureus isolate | MGH; this study |
| NCTC 8325 | Wild-type strain; rsbU mutant | 41; The Staphylococcal Genetic Stock Center; J. J. Iandolo |
| NCTC 8325-4 | Prophage-cured derivative of NCTC 8325; rsbU mutant | 63 S. J. Foster |
| RN6390 | Prophage-cured derivative of NCTC 8325 | 68; A. L. Cheung |
| RN6390B | RN6390 reisolated in the Novick laboratory with stable alpha-hemolysin production | 64; M. J. McGavin |
| COL | Wild-type Mcr strain; mec | 75; G. B. Pier |
| Newman | ATCC 25904; high level of clumping factor; σB+ | 32; M. Bischoff |
| Reynolds | Wild-type strain; type 5 capsular polysaccharide | 49; J. C. Lee |
| RN6911 | RN6390 Δagr::tetM agr sarA+ Tcr | 47; A. L. Cheung |
| ALC488 | RN6390 sarA::ermC agr+sarA Emr | 19; A. L. Cheung |
| ALC837 | RN6390 hla::ermC Hla− Emr | 45; A. L. Cheung |
| ALC842 | RN6390 Δagr::tetM sarA::ermC agr sarA Tcr Emr | 19; A. L. Cheung |
| SP6391 | RN6390B sspA::ermAB SspA− Emr | 72; M. J. McGavin |
| IK194 | Newman ΔrsbUVW sigB::ermB σB− Emr | 8; M. Bischoff |
| ALC1435 | RN6390(pALC1420) sarA P1 promoter::gfpuv | 23; A. L. Cheung |
| SH1000 | NCTC 8325-4 rsbU+ | 40; S. J. Foster |
| E. coli | ||
| OP50 | Uracil auxotrophy | 15; laboratory collection |
| DH5α(pSMC2) | DH5α containing a stable plasmid which constitutively expresses a bright mutant of A. victoria GFP; Apr | 11 |
| E. faecium | ||
| E007 | Clinical E. faecium isolate | 36 |
| B. subtilis | ||
| PY79 | Laboratory strain | 85 |
| C. elegans | ||
| Bristol N2 | Wild-type strain | Laboratory strain |
| AU1 | esp-2(ag1) | 46; D. H. Kim |
| AU3 | esp-8(ag3) | 46; D. H. Kim |
| BA1 | fer-1(hc1) | Laboratory strain |
a
Abbreviations: Mc, methicillin; Tc, tetracycline; Em, erythromycin; Ap, ampicillin; Hla, alpha-hemolysin; SspA, V8 protease.
b
MGH, Massachusetts General Hospital.