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CAS plate assay used for the detection of siderophore production. (A) E. coli strain MC4100 (parental strain); (B) E. coli strain H1780; (C) H1780 plus pFUR-3KF; (D) H1780 plus pFURK-3. The large halos in quadrants B and D represent siderophore production. This is not apparent in quadrants A and C, although there are minor halos that could be the result of heavy bacterial growth or low-level siderophore production.
