FIG. 2.
Expression cassettes to produce and secrete IL-12 in L. lactis: schematic structures of p35 and p40 subunits and scIL-12 cassette expressed under the lactococcal PnisA promoter and carried by the plasmids indicated. For details concerning plasmid construction, see the text and Table 1. The arrows indicate the presence of the nisin-inducible promoter (PnisA); the solid vertical bars indicate the RBS of the usp45 gene (bar 1 for p35) or of the exp4 gene (bar 2 for p40); the dark gray bars indicate the SP of the usp45 gene; the light gray bar indicates the SP of the exp4 gene (48); the cross-hatched bar indicates the p35 mature coding sequence; the dotted bar indicates the p40 mature coding sequence; the open bar indicates the scIL-12 coding sequence; and the stem-loop symbols indicate trpA transcription terminators (not to scale).