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. 2003 Apr;71(4):1887–1896. doi: 10.1128/IAI.71.4.1887-1896.2003

FIG. 3.

FIG. 3.

Production of IL-12 by recombinant L. lactis. IL-12 production was analyzed by immunoblotting by using anti-IL-12 antibodies. Protein samples were prepared from induced recombinant L. lactis cultures. (A) Immunodetection after SDS-PAGE. Lane C, cell fraction of the NZ(pSEC:p35-p40) strain encoding the p35 and p40 subunits (IL-12p35-p40); lane S, supernatant samples. The positions and sizes of molecular weight markers are indicated on the left. (B) Quantification of IL-12p35-p40 by ELISA (R&D Systems). STD, 80 pg of commercial murine rIL-12 per ml; IL-12p35-p40, supernatant sample of NZ(pSEC:p35-p40) culture.