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. 2003 Apr;71(4):1887–1896. doi: 10.1128/IAI.71.4.1887-1896.2003

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FIG. 4. — Secretion analysis of the two IL-12 forms produced by L. lactis. (A) rIL-12 production compared by SDS-PAGE and Western blot analysis of supernatant samples prepared from induced cultures of NZ(pSEC:p35-p40) and NZ(pSEC:scIL-12) encoding IL-12p35-p40 and scIL-12, respectively. Lane CTRL contained rIL-12 (R&D Systems) as a control. (B) Quantification of IL-12 forms produced by L. lactis by ELISA by using supernatants of induced cultures of NZ(pSEC:p35-p40) and NZ(pSEC:scIL-12) encoding IL-12p35-p40 and scIL-12. STD, 80 pg of commercial rIL-12 per ml.