FIG. 6.
Production of IFN-γ in mouse splenocytes after intranasal inoculation of recombinant L. lactis. Levels of IFN-γ were determined following sacrifice on day 35 for mice that received 5 × 108 CFU of wild-type L. lactis [L. lactis (wt)], NZ(pSEC:p35-p40), or NZ(pSEC:scIL-12) or PBS alone. The data are representative of one of three separate experiments in which similar results were obtained. The values are the means and standard deviations for three mice per treatment group. Statistically significant differences (P < 0.05) compared with the wild-type L. lactis and PBS control groups are indicated by one asterisk for the NZ(pSEC:p35-p40) group and by two asterisks for NZ(pSEC:scIL-12) group.