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. 2003 Apr;71(4):1887–1896. doi: 10.1128/IAI.71.4.1887-1896.2003

FIG. 7.

FIG. 7.

Production of Th1 cytokines by splenocytes of mice immunized with recombinant L. lactis. Levels of Th1 cytokines were determined following sacrifice on day 35 for mice immunized with 5 × 108 CFU of wild-type L. lactis (wt) or recombinant L. lactis displaying E7 antigen (E7) and for mice coimmunized with L. lactis displaying E7 and an L. lactis strain secreting active murine IL-12 (E7/IL-12). Spleen cells were cultured for 24 h with 2 μg of E7 peptide (RAHYNIVTF) (peptide) or PBS, and the levels of the Th1 cytokines IL-2 (A) and IFN-γ (B) in the culture supernatants were determined by ELISA. The values are the means and standard deviations for three mice per treatment group. Statistically significant differences (P < 0.05) compared to the E7/PBS group are indicated by one asterisk and by two asterisks for the E7/peptide and E7/IL-12/peptide groups, respectively.