. 2003 Apr;71(4):1887–1896. doi: 10.1128/IAI.71.4.1887-1896.2003

TABLE 1.

Bacterial strains and plasmids used

Strain or plasmid	Replicon	Genotype or characteristics	Reference or source
Strains
E. coli TG1		supE hsdΔ5 thi Δ(lac-proAB) F′(traD36 proAB⁺lacI lacZΔM15)	19
L. lactis MG1363		Wild type, plasmid free	14
L. lactis NZ9000		MG1363 (nisRK genes in chromosome), plasmid free	30
Plasmids
pWRG3169	ColE1	Ap^r, pBS derivative containing coding sequences for p35 and p40 subunits	49
pCR:TOPO	ori pUC	Ap^r	Invitrogen
pCR-TOPO:Δp35exon1	ori pUC	Ap^r, DNA fragment encoding first exon of p35 subunit devoid of its SP	This study
pCR-TOPO:p35exon2	ori pUC	Ap^r, DNA fragment encoding second exon of p35 subunit	This study
pCR-TOPO:Δp35	ori pUC	Ap^r, DNA fragment encoding p35 mature moiety devoid of intron	This study
pVE8001	ColE1	Ap^r, pBS derivative containing trpA transcription terminator	48
pBS:Δp40:trpA	ColE1	Ap^r, DNA fragment encoding p40 mature moiety devoid of its SP plus trpA	This study
pVE8022	ColE1/pAMβ	Ap^r Em^r, pFUN derivative containing Exp4-ΔSP_Nuc fusion	48
pBS:SP_Exp4	ColE1	Ap^r, PCR fragment encoding SP_Exp4 SP	This study
pBS:SP_Exp4:p40:trpA	ColE1	Ap^r, gene expressed from P_nisA encodes SP_Usp-p40:trpA p40 subunit precursor	This study
pSEC:E7	pWV01	Cm^r, gene expressed from P_nisA encodes SP_Usp-E7 precursor	5
pSEC:p35	pWV01	Cm^r, gene expressed from P_nisA encodes SP_Usp-p35 p35 subunit precursor	This study
pSEC:p35-p40	pWV01	Cm^r, gene expressed from P_nisA encodes SP_Usp-p35-p40 p35 and p40 subunit precursors	This study
pCDNA3:IL-12	ColE1	Ap^r, PCR fragment encoding IL-12 single chain	P. Melby^a
pCR:TOPO:scIL-12	ori pUC	Ap^r, PCR fragment encoding scIL-12 single chain devoid of its SP	This study
pSEC:scIL-12	pWV01	Cm^r, gene expressed from P_nisA encodes SP_Usp-IL-12 single-chain precursor	This study

The University of Texas Health Science Center, San Antonio.