Figure 2.

Phenotypes of transgenic tobacco plants constitutively expressing SCaM-4 or SCaM-5. (A) Formation of spontaneous disease lesion-like necrotic regions in the transgenic plants. The whole plant morphology of an 8-week-old representative transgenic SCaM-4 plant (Right) shows that lesions appeared only in the older leaves. A normal wild-type plant of similar age is shown (Left). (B) A closer look at the spontaneous lesions formed on the leaf of a 10-week-old SCaM-5 transgenic plant (Right). A normal leaf of a similar age is shown (Left) for comparison. (C and D) Accumulation of UV-excitable fluorescent material in the cell walls of spontaneously formed lesions. Microscopic examination of a spontaneous lesion under differential interference contrast (C) and epifluorecence (D) optics after nuclear staining of leaf tissues with 4′, 6-diamidino-2-phenylindole. Magnifications: ×50.