FIG. 3.

RT-PCR analysis of recombinant mFIPV. RT-PCR was used to amplify the FIPV-MF S boundaries with RNA isolated from cells infected with mFIPV as a template. (A) Physical map of the genome of the mFIPV recombinant. The dotted area indicates the MF-S gene. The location of the primers used is indicated with an arrow, together with the expected size of the PCR products. (B) RT-PCR with primers PR1254 and PR1255. (C) RT-PCR with primers PR-990 and PR311. See Table 1 for primer sequences. Lanes −RT, no reverse transcriptase added; lanes +RT, reverse transcriptase added; lanes H₂O, H₂O replacing the template; lanes Contr., pBRDI2 used as a template.