FIG. 8.

Single-cycle growth analysis. HeLa cells were infected with viruses containing wt or mutant M proteins at a multiplicity of 20 PFU/cell. At 1 h postinfection, the medium was removed, and cells were washed twice. Fresh medium was added to the infected cells, and a small aliquot of the supernatant was removed at the indicated times postinfection to determine the amount of progeny virus by plaque assay. Data are the average of two independent experiments.