Figure 1.
Schematic representation of recombinant TEV genomes. The bar, P450SU1, and uidA (GUS) genes were fused in-frame within the TEV open-reading frame (thick bar) between the coding sequences for P1 and HC-Pro proteins in the pTEV7DA-CMK cloning vector. The bar, P450SU1, and GUS polypeptides are cleaved from the nascent TEV polyprotein by two viral encoded proteinases, P1 and NIa. The pTEV7DA-CMK cloning vector contains a polylinker cloning site adjacent to an artificial NIa proteinase cleavage site (amino acid sequence given in single-letter code, with arrow indicating the scissile bond). TEV-GUS was described previously, as were the logic and principles used for vector design. The names of TEV proteins are indicated above the TEV7DA-CMK genome map. SP6, SP6 polymerase promoter for producing infectious RNA transcripts in vitro.