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. 1999 Jan 19;96(2):772–777. doi: 10.1073/pnas.96.2.772

Figure 2.

Figure 2

TEV-bar and TEV-P450 selection strategies. (A) Ten A. thaliana C24 or Col-3 plants were inoculated with TEV-bar, and inflorescences were removed at 11 days p.i., then sprayed with 0.01% GA at 11 and 18 days p.i. At 14 days after the initial GA application, C24 plants had produced new inflorescences, whereas Col-3 plants were dead. (B) Capsid protein (CP) immunoblot analysis of purified TEV capsid protein (lane 1), inflorescence tissue from a mock-inoculated C24 plant (lane 2), or inflorescence tissue from C24 and Col-3 plants inoculated with TEV-bar (lanes 3, 4) or TEV-P450 (lanes 5, 6). The TEV-bar- and TEV-P450-inoculated plants were treated with GA and R7402, respectively. (C) Sets of 10 C24 and Col-3 plants were either inoculated with TEV-P450 (3, 6) or mock-inoculated (1, 2, 4, 5). At 16 days p.i., inflorescence tissue was removed from each plant and two pots of each ecotype were sprayed with 100 μg/l R7402. At 16 days after R7402 application, C24 plants that were inoculated with TEV-P450 were severely stunted and chlorotic, whereas all other plants grew similar to nontreated control plants.