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. 1999 Jan 19;96(2):772–777. doi: 10.1073/pnas.96.2.772

Figure 3.

Figure 3

Isolation of gain-of-susceptibility mutants of A. thaliana Col-0 by using the TEV-bar selectable virus. (A) Identification of mutants A130 and A158 as survivors after GA application. The photographs were taken 13 days after GA application. (B) Capsid protein (CP) immunoblot analysis of purified TEV capsid protein (lane 1), inflorescence tissue from a mock-inoculated C24 plant (lane 2), or inflorescence tissue from C24, Col-3, or GA survivors A130, A133, and A138 inoculated with TEV-bar (lanes 3–7). (C) β-Glucuronidase (GUS) activity assays of inflorescence tissue from M3 progeny of GA survivors (A130, A133, A138, A154, and A158) or restrictive (Col-3) and susceptible (C24) control plants inoculated with TEV-GUS. GUS assays were done at 15 days p.i. or 16 days p.i. for A154 and A158.