Table 1.

Summary of mutant screens using TEV-bar

Screen	Mutagen*	Number screened	GA survivors†	Capsid positive‡	Col-0 ecotype§	M3 progeny susceptible¶
1	EMS	7,400	7	4	3	3
2	FNB	16,900	12	10	0	ND‖
3	EMS	13,600	3	1	1	1
4	EMS	12,000	5	1	1	0
5	EMS	13,250	7	6	6	6
6	EMS	13,000	19	3	1	1
7	EMS	5,000	11	3**	3	ND‖
Total		81,150	64	28	15	11

^*EMS, ethyl methanesulfonate; FNB, fast-neutron bombarded. 

^†Number of plants that regenerated inflorescence tissue after GA application. 

^‡Number of GA survivors that possessed capsid protein in newly regenerated inflorescence tissue. 

^§Number of TEV capsid protein-positive GA survivors that were verified to be of the Col-0 ecotype based on the genotype for four different SSLP markers (nga6, nga76, nga111, and nga172). 

^¶Number of capsid protein-positive Col-0 M2 GA survivors whose M3 progeny were systemically infected by TEV-GUS. These plants were considered to have heritable mutations resulting in a gain-of-susceptibility phenotype after TEV infection. 

^‖ND, not determined. 

^**In screen 7, only the three GA survivors that had the Col-0 genotype were tested for TEV capsid protein in systemic tissue. Therefore, a total of 56 GA survivors were tested for systemic movement by TEV.