Table 2.
Genetic characterization of gain-of-susceptibility mutants
| Cross | TEV-GUS infection phenotype*
|
Ratio | |
|---|---|---|---|
| Restrictive | Susceptible | ||
| Test of dominance (F1) | |||
| Col-0 × A130 | 5 | 0 | |
| Col-0 × A133 | 4 | 0 | |
| Col-0 × A138 | 5 | 0 | |
| A154 × Col-0 | 9 | 0 | |
| A158 × Col-0 | 23† | 0 | |
| A159 × Col-0 | 8 | 0 | |
| Segregation analyses (F2) | |||
| Col-0 × A130 | 41 | 12 | 3:1 (χ2 = 0.16)‡ |
| Col-0 × A133 | 71 | 23 | 3:1 (χ2 = 0.015)‡ |
| Col-0 × A138 | 105 | 32 | 3:1 (χ2 = 0.2)‡ |
| Complementation (F1) | |||
| La-er × A130 | 0 | 10 | |
| La-er × A133 | 0 | 10 | |
| La-er × A138 | 10 | 0 | |
| A154 × La-er | 0 | 12 | |
| A158 × La-er | 0 | 12 | |
| A159 × La-er | 0 | 12 | |
| A130 × A133 | 0 | 23† | |
| A130 × A138 | 17† | 0 | |
| A138 × A133 | 6 | 1§ | |
*
Plants were inoculated with TEV-GUS and inflorescence tissue was assayed at 15 and 21 days p.i. for GUS activity. All plants regardless of whether they contained GUS activity in inflorescence tissue, were confirmed to be successfully inoculated by detection of infection foci in inoculated leaves using the GUS colorimetric assay (9).
†
Data shown are combined from two reciprocal crosses.
‡
P > 0.5.
§
This plant was likely the result of accidental self-pollination of A138 at the time of crossing. All other data indicated that A138 carried a mutation that could complement rtm1 plants and that segregated as a recessive monogenic locus.