FIG. 5.

Overexpression of Bcl-2 reduces apoptosis induced by VSV in HeLa cells. (A) Quantitation of apoptosis by caspase-3 activity. The indicated HeLa cell lines were infected with rwt virus (open bars) or rM51R-M virus (solid bars) for the indicated times postinfection (pi). Uninfected samples denoted by the dashes were negative controls. Cell lysates were analyzed for caspase-3-like activity and protein concentrations as described in the legend to Fig. 2. The amount of caspase-3 activity is expressed in arbitrary fluorescence units per milligram of protein. The data represent the average ± standard deviation of three experiments. (B) Quantitation of M protein by [³⁵S]methionine labeling. The indicated HeLa cell lines were infected with rwt virus (open bars) or rM51R-M virus (solid bars) for 12 h. Cells were labeled with [³⁵S]methionine continuously throughout infection. Equal amounts of cellular proteins were analyzed by SDS-PAGE and phosphorescence imaging. Figure 5B shows quantitation of the radioactivity of the M protein bands from three separate experiments expressed as a percentage of the amount of M protein synthesis with rM51R-M in HeLa cells. The data represent the average ± standard deviation of three experiments.