FIG. 8.

The caspase-8 inhibitor does not prevent the morphological changes associated with apoptosis induced by M protein, actinomycin D, or DRB. Phase-contrast images were captured of HeLa cells transfected with M mRNA (M), or treated with TRAIL (T), actinomycin D (A), or DRB (D) in the presence of the indicated concentrations of the caspase-8 inhibitor as described in the legend to Fig. 7. HeLa cells were untreated (open bar) or transfected with yeast RNA (shaded bar) as negative controls. Between 300 and 700 cells in five representative images for each sample were scored as either flat or round. The data are expressed as the percentage of round cells and represent the average ± standard deviation of three experiments.