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. 2003 Apr;77(8):4722–4730. doi: 10.1128/JVI.77.8.4722-4730.2003

FIG. 3.

FIG. 3.

Inhibition of MuLV and PFV capsid infectivities by different lysosomotropic agents. HT1080 cells were infected with pretitrated, EGFP-expressing MuLV or PFV vectors pseudotyped by PFV glycoproteins in the presence of different concentrations of concanamycin A (A) or chloroquine (B) as indicated. Incubation with lysosomotropic agents, determination of the percentages of EGFP-expressing cells, and calculation of relative infectivities were done as described in the legend for Fig. 2. The means and standard deviations of results from 3 to 10 experiments for each of the indicated concentrations of the lysosomotropic agents are shown. Pseudotypes were generated by cotransfecting 293T cells with the following expression constructs: MuLV, pczCFG2fEGN plus pHIT60 plus pczHFVenvΔ2MuLV; PFV, pMH118 plus pczHFVenvEM02.