Northern blot analysis of difF expression. difF expression in different tissues (A) and in the corolla limb at different stages (B, lanes 1–6) of development (cf. ref. 18). (C) difF expression in the corolla limb of wild-type (R27) and mutant lines (W162, W115, and W134) for the regulators an1, an2, and an11. (D) difF mRNA in corolla limbs homozygous for the mutable alleles difF-V2082 or difF-W2090 (m/m) and wild-type (+/+) siblings. gapdh, Glyceraldehyde-3-phosphate dehydrogenase.