FIG. 3.

CMV-induced cytokine secretion is CD14 and TLR2 dependent. (A) HEK293 cells stably transfected with CD14 alone or in combination with TLR2 or TLR4 were incubated with no stimulus (Mock) or with IL-1β (100 ng/ml; TLR- and CD14-independent positive control ligand), phenol-reextracted LPS (pLPS; 10 ng/ml; TLR4 ligand), yeast zymosan (10 μg/ml; TLR2 ligand), or virions, UV-inactivated virions, or DBs. The supernatants were harvested 18 h later, and IL-8 levels were determined by ELISA. The error bars indicate standard deviations. (B) PECs (10⁵/well) isolated from thioglycolate-injected wild-type (wt), TLR2^−/−, or TLR4^−/− mice were incubated with no stimulus (Medium) or with pLPS (10 ng/ml; TLR4 ligand), yeast zymosan (10 μg/ml; TLR2 ligand), or UV-inactivated CMV (1 × 10³ to 3 × 10⁴ PFU/well). The supernatants were harvested 18 h later, and IL-6 levels were determined by ELISA.