FIG. 5.

TLR2 is required for NF-κB activation in response to CMV. (A and B) CHO/CD14 (A) or CHO/CD14/TLR2 (B) reporter cells were mock infected, treated with zymosan A (10 μg/ml), or infected with CMV (multiplicity of infection, 0.1), and NF-κB activity was measured using the NF-κB-driven CD25 reporter. At 18 h posttreatment-infection, cell surface expression of CD25 was measured by FACS. For each histogram, the vertical axis represents the relative cell numbers, and the horizontal axis represents the intensity of fluorescence staining. The shaded areas represent the isotype control, the dotted lines represent mock-infected cells, the thin solid lines represent zymosan A-treated cells, and the thick solid lines represent CMV-infected cells. IgG, immunoglobulin G; FITC, fluorescein isothiocyanate. (C) The percentages of CD25-positive cells from the data in panels A and B are presented graphically.