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TCD8+ response of B6 mice to boiled virus. Splenocytes or tPEC from B6 mice immunized with infectious (A) or boiled (B) NT60 were tested ex vivo by ICS for recognition of DC2.4 cells sensitized with the indicated peptides or with infectious NT60. Note that the response elicited by infectious NT60 was ∼20-fold higher than that elicited by bNT60. Similar results were obtained in four additional experiments.
