FIG. 3.
Presentation of bNT60 to NP366-374-specific TCD8+ in vitro. (A) The indicated cells were incubated with bNT60 for 6 h prior to addition of an NP366-374-specific TCD8+ line. After 3 h, the activation of TCD8+ was assessed by ICS. BMDC, bone marrow-derived dendritic cells; BMMΦ, bone marrow macrophages. (B) tPEC incubated as in panel A with supernatants from bNT60 after centrifugation for 10 min at the indicated relative centrifugal force (rcf) were used to stimulate NP366-374-specific TCD8+. (C and D) Cells were incubated with bNT60 or infectious NT60 as indicated and used to stimulate NP366-374-specific TCD8+. Similar results were obtained in an additional experiment.