Skip to main content
. 2003 Apr;77(8):4899–4910. doi: 10.1128/JVI.77.8.4899-4910.2003

TABLE 2.

Real-time RT-PCR analysis

Gene namea Real-time RT-PCR Ctb in:
Ct differencec Fold change in expression by:
APV-infected cells Uninfected cells PCR Microarray analysis
Signal transducer and activator of transcription 1-α/β 18.27 22.39 4.12 17.39 54.86
IFN-induced 56-kDa protein 15.24 23.1 7.86 232.32 20.90
IFN-induced protein with TPR 4 16.14 24.76 8.62 393.44 6.63
CXC chemokine 9E3 (human IL-8 homologue) 24.82 26.38 1.56 2.95 53.84
IFN-induced GTP-binding protein Mx 17.55 26.36 8.81 448.82 61.38
IFN-induced protein 6-16 precursor 15.48 25.7 10.22 1192.69 30.64
IFN-α-inducible protein P27-H 16.01 26.2 10.19 1168.14 23.67
Ubiquitin carboxyl-terminal hydrolase 18 (Ubp) 16.9 22.89 5.99 63.56 11.83
Complement C3F 17.99 19.31 1.32 2.50 12.91
CD80-like protein precursor 20.68 22.4 1.72 3.29 4.67
Ornithokinin receptor 29.03 33.44 4.41 21.26 2.98
Collagen alpha 2(I) chain precursor 15.18 14.32 −0.86 −1.82 −4.32
Cullin homologue 1 (CUL-1) 23.21 23.51 0.3 1.23 2.13
Lumican precursor (keratan sulfate proteoglycan) 15.81 14.35 −1.46 −2.75 −4.93
Thrombospondin 2 precursor 16.91 14.45 −2.46 −5.50 −8.24
Similar to sequestosome 1 16.05 18.71 2.66 6.32 7.78
TATA element modulatory factor (TMF) 19.58 20.6 1.02 2.03 4.83
Elongation factor 1-alpha 1 14.76 14.38 −0.38 −1.30 −1.57
Transcriptional repressor protein YY1 (Yin and Yang 1) 21.84 22.59 0.75 1.68 1.89
Ring finger protein 6 20.41 21.14 0.73 1.66 4.38
a

Genes validated by RT-PCR are also marked and listed in Table 1, except for the transcriptional repressor protein YY1 gene, which was found to be unaltered in expression by both microarray analysis and RT-PCR.

b

Threshold cycle (Ct) values are shown for the PCR amplification curves of a selected group of genes in APV-infected versus uninfected host cell mRNA at 96 h p.i. generated by a SYBR green-based RT-PCR detection system. Ct is defined as the fractional cycle number at which the fluorescence passes the fixed threshold, which is set at the start of exponential growth of the PCR product.

c

Cycle difference in the onset of exponential amplification of the target sequence.