FIG. 7.

EIAV YPDL-type L-domain activity is inhibited by catalytically inactive VPS4. (A) 293T cells were transfected with an L-domain-defective HIV-1 proviral plasmid, (pNLδp6) and a complementing Gagδp6 expression vector that expressed either no L domain (None), HIV-1 p6, or EIAV p9 fused at its C terminus, as indicated. Either no VPS4 or wild-type (WT) or DN mutant forms of VPS4 were coexpressed. Infectious HIV-1 virion production was measured by inoculation of P4/R5 indicator cells, and β-galactosidase (β-Gal) activity in lysates was measured 48 h later. The mean and standard deviation are shown. R.L.U., relative light units. (B) Western blot analysis of cell lysates and extracellular HIV-1 virion production by 293T cells transfected with the same constructs as for panel A, using an anti-HIV-1 CA antibody.