FIG. 3.
NEM-resistant disulfide-linked homodimer formation between recombinant N proteins. Marc-145 cells infected with the vaccinia virus recombinant VV-N were pulse-labeled with [35S]methionine and chased for the indicated times. The cells were solubilized in the presence of 20 mM NEM, and the N protein was immunoprecipitated using N-specific MAbs. The precipitated proteins were resolved by SDS-PAGE under reducing (+βME) (lanes 1 to 6) and nonreducing (−βME) (lanes 7 to 12) conditions and visualized using a PhosphorImager.