FIG. 5.

IL-10 treatment of microglial cells is associated with decreased CMV-induced activation of NF-κB but not phosphorylation of p38 MAP kinase. (A) Primary human microglial cells were treated overnight with IL-10 (30 ng/ml) or IL-4 (30 ng/ml) and then stimulated with sucrose-purified CMV. Activation of microglial cell NF-κB was quantified 1.5 h p.i. with an NF-κB ELISA. **, P < 0.01 versus unstimulated microglial cells; ††, P < 0.01 versus stimulated microglial cells. OD, optical density. (B) Microglial cells maintained in serum-free medium were treated with the indicated anti-inflammatory cytokine prior to viral (CMV) infection or addition of medium alone (uninfected). Whole-cell lysates were obtained for analysis of phosphorylated p38 MAP kinase (phosphorylated) and total p38 (total) by Western blot assay. C, extract obtained from uninfected microglial cells.