FIG. 4.

Staining of TG sections with the cleaved caspase-3 antibody. Thin sections were prepared from TG of calves infected with wt BHV-1 or the LR mutant. Immunohistochemistry was performed with the cleaved caspase-3 antibody. Shown are a section from a mock-infected calf (A), a TG section prepared from a calf infected with wt BHV-1 for 6 days (B), a TG section prepared from a calf infected with LR mutant for 6 days (C), a TG section prepared from a calf infected with wt BHV-1 for 14 days (D), and a TG section prepared from a calf infected with the LR mutant for 14 days (E). These sections are representative of the results from many sections. The TG from two calves were examined for each time point. The arrows denote caspase-3⁺ neurons.