Table I.

Subcellular localization of GFP-AtE2F and -DP proteins^a

Construct		Subcellular Localizationb
E2F	DP	N	C	N+C
		%
GF-AF1	–	3	0	97
GF-AF1	DPa	94	0	6
GF-AF1	DPb	0	0	100
GF-AF2	–	7	29	64
GF-AF2	DPa	11	6	83
GF-AF2	DPb	4	4	92
GF-AF3	–	3	48	49
GF-AF3	DPa	82	3	15
GF-AF3	DPb	6	36	58
–	GF-DPa	0	0	100
AF3	GF-DPa	69	0	31
–	GF-DPb	0	0	100
AF3	GF-DPb	1	0	99
GF-AF3ΔD	–	67c	0	33
GF-AF3ΔD	DPa	66c	0	64

^a Cultured tobacco cells were transfected with 0.5 mg of the indicated expression constructs of GFP fusion proteins or together with 0.5 μg of the expression constructs containing no GFP coding region. Plasmid quantities were equalized with the p35S-nptII plasmid. One hundred to 200 GFP-positive cells were scored for protein localization. 

^b Percentage of cells displaying exclusive nuclear (N), relatively cytoplasmic (C), or both nuclear and cytoplasmic fluorescence (both). 

^c The extent of the nuclear localization was weaker than that of GF-AF3 (or GF-AF1) plus DPa.