Figure 3.

Target enzyme activity in plants transformed with CO or COC partial sense chimeric constructs. The activities of CAD and COMT were measured in wild-type plants to determine the normal mean activity (100%) for each enzyme (n = 10, young plants; n = 3, mature plants). CAD and COMT activities were assayed in individual young CO (a) or COC (b) plants. Selected plants were clonally propagated to yield small populations (“lines”) of genetically identical individuals. c, Clonal plant lines were grown to maturity and assayed for CAD, COMT, and CCR (as appropriate). Error bars in c indicate the se between assays on different clonal individuals of the same plant line. d, Enzyme assay data for CAD and COMT activity in mature plants were confirmed by western blotting.