Figure 6.

DGDG synthesis in chloroplasts of Arabidopsis wild type and dgd1 mutant. A, Chloroplasts of Arabidopsis wild type and dgd1 mutant were isolated from protoplasts and incubated with MGDG and UDP-[¹⁴C]Gal. After extraction, lipids were separated by thin-layer chromatography and radiolabeled lipids visualized by autoradiography. B, Chloroplasts of wild type and dgd1 were isolated from protoplasts, lipids extracted, and stained with iodine. C, Chloroplasts of wild type and dgd1 were isolated by homogenization of leaves (Price et al., 1994), lipids extracted, and stained with iodine. Galactolipids and sulfolipid were identified by staining with α-naphthol (not shown).