Table II.
Changes in DGDG lipid content and fatty acid composition of DGDG in fad3,dgd1 double mutants after phosphate deprivation
| Lipid | WT
|
dgd1
|
fad3
|
fad3,dgd1
|
||||
|---|---|---|---|---|---|---|---|---|
| + | – | + | – | + | – | + | – | |
| DGDG | 11.8 | 23.0 | 0.9 | 9.3 | 12.9 | 26.2 | 1.1 | 9.5 |
| 16:0 | 15.4 | 21.8 | 26.0 | 29.8 | 15.3 | 16.0 | 22.7 | 22.5 |
| 16:1 | 2.1 | 1.6 | 4.2 | 2.3 | 2.2 | 1.4 | 2.4 | 0.7 |
| 16:2 | 0.9 | n.d. | 1.6 | 0.3 | 0.7 | 0.2 | 3.1 | n.d. |
| 16:3 | 2.6 | 1.9 | 3.4 | 1.1 | 3.4 | 2.5 | 1.9 | 1.8 |
| 18:0 | 1.2 | 2.5 | 3.7 | 5.3 | 4.5 | 2.4 | 2.1 | 3.1 |
| 18:1 | 2.0 | 2.9 | 6.4 | 4.9 | 3.2 | 2.3 | 5.4 | 2.3 |
| 18:2 | 4.9 | 10.8 | 9.2 | 12.5 | 7.1 | 12.2 | 9.2 | 15.6 |
| 18:3 | 70.9 | 58.6 | 45.2 | 44.0 | 63.7 | 63.0 | 53.2 | 53.4 |
Values are given as mol % and represent averages of three measurements. se error was less than 2% for all measurements. Plants were grown on solidified medium supplemented with Suc containing 1 mm (+) or no phosphate (−). n.d., Not detected.