Table I.
Characteristics of ESTs analyzed
| Project | Source | No. of ESTs | Average Length | Average Phred Quality Score | Source-Specific ESTs |
|---|---|---|---|---|---|
| nt | % | ||||
| 486 | Leaf primordia | 5,867 | 386 | N.D. | 35 |
| 605 | 10- to 14-d-old endosperm | 6,566 | 510 | 30.9 | 25 |
| 606 | 1- to 2-cm immature ear | 5,518 | 526 | 32.9 | 24 |
| 614 | 4-d-old root | 10,635 | 460 | 43.3 | 34 |
| 618 | <2-cm tassel | 3,407 | 378 | 45.3 | 27 |
| 660 | Mixed tassel stages | 6,444 | 444 | 45.1 | 31 |
| 687 | Mixed embryo stages | 4,765 | 458 | 45.2 | 33 |
| 707 | Mixed adult organs | 8,688 | 439 | 41.4 | 43 |
| 946 | 1-mm tassel | 10,646 | 412 | 38.9 | 26 |
All EST sequences were submitted to GenBank and are also available with annotation at ZmDB (http://zmdb.iastate.edu). Minimal requirements for submission were a sequence length of at least 100 consecutive nucleotides (nt) with a minimum Phred quality score of 15 in each position for sequences determined with capillary electrophoresis technology. N.D., Not determined because 486 samples were sequenced using both gel and capillary electrophoresis technologies. The percentage of source-specific ESTs was calculated as the fraction of ESTs within each project that formed singlets or contigs with ESTs exclusively from the same project after assembly of all ESTs from all projects.