Figure 8.

Comparison of LeExt1 and Rpl2 transcript levels in tomato tissues by RT-PCR. RT-PCR was performed with total RNA from roots, leaves, and anthers and hybridized with randomly labeled cDNA probes, as indicated at left. RNA from leaves of transgenic plants constitutively expressing LeExt1 served as a control for primer specificity (control). RNA samples that were not subjected to reverse transcription (−rt) served as a control to determine residual amounts of genomic DNA in the samples (absent in −rt treatments). Black columns indicate relative abundance of transcripts as determined by phosphorimager analysis. The signal for root RNA was arbitrarily set to 100%.