Fig. 2.

RT-PCR of claudin mRNA expression in NHU cells and the effect of TZ and PD153035 on claudin expression. NHU cells were pretreated for 24 h in the absence or presence of TZ (1 μM) and incubated in media with or without PD153035 (1 μM). Media were changed every 3 days with fresh PD153035 added. RNA was exacted at 1, 3 and 6 days, cDNA was generated and RT-PCR was performed as outlined in the methods, using claudin primers and GAPDH as the internal control. The PCR products were electrophoresed on a 2% agarose gel and visualised using ethidium bromide. Similar results were obtained with a second independent NHU cell line.