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. Author manuscript; available in PMC: 2007 Aug 1.

Published in final edited form as: J Cell Physiol. 2006 Aug;208(2):407–417. doi: 10.1002/jcp.20676

Fig. 4 — Influence of TZ and PD153035 on the localisation of the tight junction proteins. NHU cells were seeded at 2 × 10⁵ cells/ml onto glass slides, allowed to adhere and treated with or without TZ (1 μM) for 24 h. Subsequently, cells were treated in the absence or presence of PD153035 (1 μM) and slides were fixed after 1, 3 and 6 days. Media were replaced every 3 days with fresh PD153035 added. Immunofluorescence was performed using the antibodies indicated. The images depicted in the figure are from slides fixed at 6 days. Scale bar, 30 μm. Similar results were obtained in a further two independent NHU cell lines.

Fig. 4 — Influence of TZ and PD153035 on the localisation of the tight junction proteins. NHU cells were seeded at 2 × 10⁵ cells/ml onto glass slides, allowed to adhere and treated with or without TZ (1 μM) for 24 h. Subsequently, cells were treated in the absence or presence of PD153035 (1 μM) and slides were fixed after 1, 3 and 6 days. Media were replaced every 3 days with fresh PD153035 added. Immunofluorescence was performed using the antibodies indicated. The images depicted in the figure are from slides fixed at 6 days. Scale bar, 30 μm. Similar results were obtained in a further two independent NHU cell lines.