TABLE 2.
Summary of claudin expression patterns
| Claudin
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Method | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 10 | ||
| In situ | RT-PCR | + | + | −/+ | + | + | − | + | −/+ | −/+ | |
| IHC | NA | NA | + | + | + | NA | + | NA | NA | ||
| In vitro:-baseline | RT-PCR | + | + | + | + | + | − | + | −/+ | − | |
| WB | + | NA | −/+ | + | − | NA | + | NA | NA | ||
| In vitro: differentiating (+TZ +PD153035) | RT-PCR | = | ↘ | ↑ | = | ↘ | − | = | −/= | − | |
| WB | ↘ | NA | ↑ | ↑ | ↑ | NA | ↗ | NA | NA | ||
NA, no antibody available; ↑, major increase after treatment; ↘, small decrease after treatment; ↗, small increase after treatment; =, expression stays constant after treatment; +, present; −, absent; −/+, −/=, variable expression.