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Assay for the presence of the GL8 protein in GA-enriched fraction. A, Immunoblot analysis of the GL8 protein in the ER-enriched fraction (ER), microsomal fraction after the removal of the GA (Ms-GA), and the GA-enriched fraction (GA). Eighty micrograms of protein was loaded per lane. B, IDPase activity associated with the membrane fractions described above. The open and filled bars represent IDPase activity in the absence and presence of Triton X-100, respectively.
