Skip to main content
. 2006 Jul 15;20(14):1885–1898. doi: 10.1101/gad.1424106

Figure 6.

Figure 6.

mRNA decay triggered by miRNAs occurs by a deadenylation and decapping mechanism requiring GW182. (A,B) S2 cells depleted of GFP, AGO1, GW182, CAF1, NOT1, or the DCP1:DCP2 decapping complex were transfected with plasmids expressing the miRNA reporters described in Figure 5E. Firefly luciferase activity and the corresponding mRNA levels were measured and normalized to those of the Renilla control. Normalized firefly luciferase activities and mRNA levels in cells transfected with the empty vector (black bars) were set to 100% for each knockdown. Error bars represent standard deviations from three independent experiments. (C,D) Northern blot of representative samples shown in A and B. (E,F) RNA samples shown in C and D, lanes corresponding to the DCP1 + 2 knockdown, were treated with RNase H in the absence or presence of oligo(dT) and analyzed by Northern blot. (G,H) S2 cells treated with dsRNAs targeting GFP or AGO1 were transfected with plasmids for the λN-tethering assay. Firefly luciferase activity and the corresponding mRNA levels were measured and normalized to those of the Renilla control as described in Figure 5. (H) Northern blot of representative mRNA samples shown in G.