Figure 3.

Confirmation of proACS activity across chromosome VII. (A) Schematic representation of chromosome VII showing the locations of proACS elements with ARS activity that we have verified (colored vertical bars). The origins in the 14 regions predicted by all three previous studies (Raghuraman et al. 2001; Wyrick et al. 2001; Yabuki et al. 2002) are shown as red bars. Origins at locations identified by only one or two of the previous studies are shown as orange bars. For representations of other chromosomes, see Supplementary Figure S4. The centromere is indicated by a black box and the telomeres are indicated by zigzags. (B) Representative ARS assay plates for a subset of the chromosome VII origins tested. (C) Results of ARS assays when the proACS is mutated.