Figure 6.

NijA and Mmp1 colocalize to cell surfaces in trachea. A–D are images of one sample; E–F are images of another sample. (A) NijA whole serum recognizes cell surfaces in tracheal dorsal trunks. (B) Mmp1 antibodies also recognize cell surfaces in dorsal trunks. (C) DAPI staining of nuclei in the same sample. (D) A merged image of the images in A–C showing the colocalization of NijA and Mmp1. (E) The cell surface staining is blocked by preabsorbing the NijA antibody with the antigenic peptide, demonstrating the specificity of the antibody in fixed tissue. (F) Mmp1 staining is unaffected by antibody preabsorption with the NijA antigenic peptide.